肝细胞癌中载脂蛋白M,肝受体同系物1和肝细胞核因子1α表达的相关性分析

Expressions of apolipoprotein M,liver receptor homolog-1 and hepatocyte nuclear factor-1α in hepatocellular carcinoma

目的:探讨肝细胞癌(HCC)组织和癌旁组织中载脂蛋白M(apoM)、肝受体同系物1(LRH-1)和肝细胞核因子1α(HNF-1α)的表达并分析3者之间的相关性。方法:采用RT-PCR和免疫组化分别检测17例原发性肝细胞癌和相应癌旁组织中apoM、LRH-1、HNF-1αmRNA的表达及apoM和HNF-1α蛋白的表达。结果:apoM、LRH-1和HNF-1αmRNA及apoM和HNF-1α蛋白在癌组织中的表达均明显高于癌旁组织(P均<0.01)。LRH-1、HNF-1αmRNA和apoMmRNA表达均呈正相关关系(依次为r=0.463,P<0.01;r=0.356,P<0.05)。结论:肝细胞癌中apoM的表达和LRH-1及HNF-1α表达密切相关,LRH-1和HNF-1α可能是apoM表达的重要调节因子。

OBJECTIVE：To explore the expressions of apolipoprotein M （apoM）, liver receptor homolog-1（LRH-1） and hepatocyte nuclear factor-1α（HNF-1α） in hepatocellular carcinoma（HCC）tissue and para-cancer tissue,and analyze the associations among them. METHODS： The expressions of apoM,LRH-1, HNF-1α at the level of mRNA and apoM,HNF-1α at the level of protein were evaluated by RT-PCR and immunohistochemistry in the 17 primary HCC and para-cancer tissues. RESULTS： The expressions of apoM, LRH-1, HNF-1α mRNA and apoM,HNF-1α protein were obviously higher in HCC tissue than that in para-cancer tissue （P0.01）. LRH-1,HNF-1α showed significant positive correlation with the expression of apoM mRNA （r=0.463,P0.01;r=0.356,P0.05）. CONCLUSION： Expression of apoM had close correlation with the expressions of LRH-1 and HNF-1α in HCC, and LRH-1 and HNF-1α may be the important regulatory factors of the apoM .