摘要
目的:探讨肝细胞癌(HCC)组织和癌旁组织中载脂蛋白M(apoM)、肝受体同系物1(LRH-1)和肝细胞核因子1α(HNF-1α)的表达并分析3者之间的相关性。方法:采用RT-PCR和免疫组化分别检测17例原发性肝细胞癌和相应癌旁组织中apoM、LRH-1、HNF-1αmRNA的表达及apoM和HNF-1α蛋白的表达。结果:apoM、LRH-1和HNF-1αmRNA及apoM和HNF-1α蛋白在癌组织中的表达均明显高于癌旁组织(P均<0.01)。LRH-1、HNF-1αmRNA和apoMmRNA表达均呈正相关关系(依次为r=0.463,P<0.01;r=0.356,P<0.05)。结论:肝细胞癌中apoM的表达和LRH-1及HNF-1α表达密切相关,LRH-1和HNF-1α可能是apoM表达的重要调节因子。
OBJECTIVE:To explore the expressions of apolipoprotein M (apoM), liver receptor homolog-1(LRH-1) and hepatocyte nuclear factor-1α(HNF-1α) in hepatocellular carcinoma(HCC)tissue and para-cancer tissue,and analyze the associations among them. METHODS: The expressions of apoM,LRH-1, HNF-1α at the level of mRNA and apoM,HNF-1α at the level of protein were evaluated by RT-PCR and immunohistochemistry in the 17 primary HCC and para-cancer tissues. RESULTS: The expressions of apoM, LRH-1, HNF-1α mRNA and apoM,HNF-1α protein were obviously higher in HCC tissue than that in para-cancer tissue (P0.01). LRH-1,HNF-1α showed significant positive correlation with the expression of apoM mRNA (r=0.463,P0.01;r=0.356,P0.05). CONCLUSION: Expression of apoM had close correlation with the expressions of LRH-1 and HNF-1α in HCC, and LRH-1 and HNF-1α may be the important regulatory factors of the apoM .
出处
《癌变.畸变.突变》
CAS
CSCD
2010年第2期130-133,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
安徽省自然科学基金项目(090413111)
关键词
肝细胞癌
载脂蛋白M
肝受体同系物1
肝细胞核因子1α
hepatocellular carcinoma
apolipoprotein M
liver receptor homolog-1
hepatocyte nuclear factor-1α