Ezrin、E-cadherin在食管鳞癌中的表达及临床意义

Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma

背景与目的:有研究表明膜细胞骨架组织连接蛋白埃兹蛋白(Ezrin)能够促进肿瘤转移,可与上皮钙黏素相互作用,参与肿瘤细胞的转移过程。本研究探讨Ezrin和E-cadherin在食管鳞状细胞癌中的表达及与临床病理因素之间的关系,分析其在食管鳞癌中的诊断价值。方法:应用组织芯片技术免疫组织化学法(SP法)检测76例食管鳞状细胞癌患者组织标本及癌旁正常鳞状上皮中Ezrin和E-cadherin的表达情况,结合临床相关因素进行卡方检验及非参数检验等统计学分析。结果:76例食管癌旁正常鳞状上皮中Ezrin阳性35例(46.1%),E-cadherin阳性74例(97.4%);76例食管鳞癌组织中Ezrin阳性63例(82.9%),E-cadherin阳性34例(44.7%)。Ezrin表达与食管鳞癌的浸润深度和淋巴结转移显著相关(P<0.05);E-cadherin与肿瘤分化程度和淋巴结转移显著相关(P<0.05),随肿瘤分化程度的降低,E-cadherin的表达缺失率逐渐增加。Ezrin表达升高和E-cadherin的表达缺失呈负相关。结论:Ezrin和E-cadherin均与食管鳞癌的发生、转移有关,且两者表达呈负相关。利用组织芯片技术联合检测Ezrin、E-cadherin的表达,有助于综合判断食管癌的恶性程度和转移潜能。

Background and Objective： It has been proven that Ezrin protein may interact with E-cadherin protein and take part in metastasis of tumor cells. This study was to investigate the expressions of Ezrin and E-cadherin in esophageal squamous cell carcinoma （ESCC） and their relationship with the clinicopathologic factors,and to analyze their diagnostic values for ESCC. Method： The expression of Ezrin and E-cadherin in 72 specimen of ESCC and the para-cancer normal squamous epithelium was detected using tissue array with SP immunohistochemistry. Their correlations with the clinicopathologic factors were analyzed statistically. Results： The positive rate of Ezrin was significantly higher in ESCC than in para-cancer normal squamous epithelium （90.7% vs. 46.0%,P〈0.001）; the positive rate of E-cadherin was significantly lower in ESCC than in para-cancer normal squamous epithelium （27.6% vs. 97.4%,P〈0.001）. Ezrin expression was related to the invasiveness and lymph node metastasis of ESCC （ P〈0.05）; E-cadherin expression was related to the differentiation and lymph node metastasis of ESCC （ P〈0.05）. The high expression of Ezrin was related to the low expression of E-cadherin （ P〈0.05）. Conclusion： The activation of Ezrin and the absence of E-cadherin contributes to the tumorigenesis and metastasis of ESCC.