尾叶桉的不同类型愈伤组织芽分化与某些相关生理生化指标的关系

Relationship between Bud Differentiation and Several Physiological and Biochemical Indexes of Three Types of Eucalyptus urophylla S.T.Blake Callus

切取8d苗龄的尾叶桉幼苗下胚轴,培养7d后愈伤组织的诱导率达100%。8周后,愈伤组织分化成褐色、白色和浅绿色3种类型。其中只有浅绿色愈伤组织能分化出不定芽,分化率达57%。浅绿色愈伤组织的过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和多酚氧化酶(PPO)的活性最高。除SOD外,浅绿色愈伤组织的POD、CAT和PPO活性与其他两类愈伤组织相比具有显著性差异(P<0.05)。H2O2和丙二醛含量则是褐色愈伤组织的最高。

Hypocotyls excised from 8 day-old seedlings could dedifferentiate and form callus at 100% when they were incubated on a modified MS （SPCa） medium for 7 days.Fifty-six days later,the callus differentiated into 3 types,including brown callus,white callus and reseda callus.Only could the reseda callus formed adventitious bud at 57% when these calli inoculated with the bud induction medium.The activities of POD,SOD,CAT and PPO of reseda callus were the highest.Compared with the enzyme activity of other types of callus,the POD,CAT and PPO activities of reseda callus showed significantly difference at 0.05 level,except for the activity of SOD.The content of H2O2 and malonaldehyde of brown callus was the highest.