摘要
采用双向凝胶电泳技术分析紫萍叶状体衰老期间内肽酶同工酶的变化以及外源L-丝氨酸对内肽酶影响的结果表明,在衰老的紫萍叶状体中检测到9种内肽酶同工酶,丝氨酸内肽酶EP3可能在叶状体衰老的早期起作用,而半胱氨酸内肽酶EP9在第6天出现,是衰老后期活性最强的内肽酶。培养液中加入外源的L-丝氨酸后,叶状体蛋白质含量下降加剧,与衰老相关的内肽酶EP3、EP4和EP9的活性明显增强或提前出现。
Changes in endopeptidase isoenzymes of Spirodela polyrrhiza thallus during senescence and L-serine induced senescence were investigated by two-dimensional electrophoresis. Nine endopeptidases were detected. Changes of these enzymes activities during senescence showed that EP3 （a serine protease） might play a significant role in early stage and the EP9 （a cysteine protease）, appeared on the 6th day, was the most active endopeptidase in the late stages of senescence. L-serine treatment of the thallus enhanced the appearance and increase in the activities of three senescence-related endopeptidases （EP3, EP4, EP9）, along with its significant promotion of decline in protein content.
出处
《植物生理学通讯》
CAS
CSCD
北大核心
2010年第3期241-244,共4页
Plant Physiology Communications
基金
国家自然科学基金(30870185)
关键词
紫萍
叶状体
衰老
内肽酶
L-丝氨酸
Spirodela polyrrhiza
thallus
senescence
endopeptidase
L-serine
