利用Imaging-PAM研究莱茵衣藻对环境变化的响应

Research of Physiological Responses of Chlamydomonas reinhardtii to Environmental Changes Utilizing Imaging-PAM

本文介绍了Imaging-PAM-M-Series调制叶绿素荧光成像技术在莱茵衣藻活体叶绿素荧光检测中的应用。该方法利用CCD对藻体直接成像,可同时检测多个样品。后期分析可获得图像中任意区域的初始荧光产量(Fo)、充分暗适应后PSII的最大光化学效率(Fv/Fm)、PSII光化学能量转化的有效量子产量[Y(II)]、光化学淬灭系数(qP)、非光化学淬灭系数(NPQ)等指标。文章以敌草隆[3-(3,4-dichlorophenyl)-1,1-dimethylurea,DCMU]和没食子酸丙酯(propyl gallate,PG)对莱茵衣藻野生型及其抗DCMU突变体的影响为例,说明该技术在莱茵衣藻研究中是可靠的,具有简单、快速、灵敏等特点。

The application of lmaging-PAM in the study of physiological responses of Chlamydomonas reinhardtii to environmental stresses was introduced. This technique can image several samples immediately and simultaneously. With this technique the minimum fluorescence yield （Fo）, the maximal efficiency of PSⅡ in a dark-adapted state （Fv/Fm）, the effective quantum yield of PSII [Y（Ⅱ）], the photochemical quenching parameter （qp） and the nonphotochemical quenching parameter （NPQ） can be monitored. A case with the effect of DCMU [3-（3,4-dichlorophenyl）- 1,1-dimethylurea] and PG （propyl gallate） on Chlamydomonas reinhardii wide-type strain and its DCMU-resistant mutant was carried. It demonstrated that this technique was reliable, simple, rapid and sensitive.