美洛昔康对人鼻咽癌CNE-1细胞株的作用

Effect of Meloxicam on nasopharygeal carcinoma CNE-1 cell line

目的探讨美洛昔康对鼻咽癌细胞株CNE-1生长抑制及诱导凋亡的作用。方法应用肿瘤细胞培养技术,随机分组和空白对照设计,培养不同时间后,用MTT法、AO+EB染色法、流式细胞仪检测法,观察美洛昔康对CNE-1生长抑制的剂量和时间效应,分析细胞凋亡发生率,细胞免疫荧光法检测细胞内COX-2蛋白的表达。结果美洛昔康能抑制CNE-1细胞的生长。美洛昔康处理CNE-1细胞后,细胞呈凋亡特征性改变。流式细胞分析显示美洛昔康呈浓度依赖性诱导CNE-1细胞凋亡。细胞免疫荧光结果表明美洛昔康处理细胞后,随药物浓度的增加,COX-2蛋白表达下降。结论美洛昔康具有抑制CNE-1细胞生长,诱导CNE-1细胞凋亡的作用,其机制可能与COX-2蛋白有关。

OBJECTIVE To study the effect of Meloxicam on the growth inhibition and apoptosis induction in nasopharyngeal carcinoma CNE-1 cell line.METHODS Technique of cell culture and randomized blank-contrast design were used.The degree and dose-dependency of Meloxicam which induced growth inhibition and apoptosis in CNE-1 cell line were observed by MTT method,AO＋EB straining and flow cytomethy.COX-2 were determined using cell immunoflurorescence.RESULTS MTT assay showed that Meloxicam inhibite the growth of CNE-1 cells.AO＋EB straining showed partial cells presented characteristic morphological changes of apoptosis.Flow cytometry analysis showed that treating CNE-1 cells with Meloxicam increase the percentage of apoptosis cells.Cell immunoflurorescence revealed that the COX-2 expression was decreased.CONCLUSION Meloxicam could significantly inhibit the growth and induce apoptosis of CNE-1 cells.Apoptosis of tumor cells is closely associated with down regulation of the ratio of COX-2.