摘要
介绍研究的最近的抄写在真核细胞的染色体揭示了 antisense 抄本的一个出人意料地大的比例。这些 antisense 基因似乎由与感觉基因交往调整基因表示。以前的研究集中了于非编码的 antisense 基因，但是 antisense 蛋白质的可能的规章的角色糟糕被理解。在这研究，我们发现那基因 ADF1 作为抄写 suppressor 扮演的 antisense 编码的蛋白质，调整在 Saccharomyces cerevisiae 的感觉基因 MDF1 的表示。基于进化，基因， cytological 和生物化学的证据，我们证明编码蛋白质的感觉基因 MDF1 很可能发源从一个以前非编码的序列和罐头的 de novo 显著地在由有约束力的地席伪 2 并且这样中等的富人压制面包师的酵母的交配效率支持植物的生长。这些结果在几个重要问题上打开新灯，包括新 sense-antisense 相互作用机制，功能的基因的 de novo 起源，和交配小径的酵母的规定。
Recent transcription profiling studies have revealed an unexpectedly large proportion of antisense transcripts in eukaryotic genomes. These antisense genes seem to regulate gene expression by interacting with sense genes. Previ- ous studies have focused on the non-coding antisense genes, but the possible regulatory role of the antisense protein is poorly understood. In this study, we found that a protein encoded by the antisense gene ADF1 acts as a transcription suppressor, regulating the expression of sense gene MDF1 in Saccharomyces cerevisiae. Based on the evolutionary, ge- netic, cytological and biochemical evidence, we show that the protein-coding sense gene MDF1 most likely originated de novo from a previously non-coding sequence and can significantly suppress the mating efficiency of baker＇s yeast in rich medium by binding MATa2 and thus promote vegetative growth. These results shed new light on several im- portant issues, including a new sense-antisense interaction mechanism, the de novo origination of a functional gene, and the regulation of yeast mating pathway.
