腰椎间盘突出症中自身体液免疫异常的实验研究

Experimental study on the abnormal humoral autoimmunity in lumbar disc herniation model

目的从动物实验的角度探讨腰椎间盘突出症中自身体液免疫异常。方法在SD大鼠身上建立非机械压迫性腰椎间盘突出症模型,运用透射比浊法和免疫组织化学染色针对大鼠外周血免疫球蛋白IgG、IgM和髓核中抗原抗体复合物沉积进行检测,并用HE染色观察大鼠神经根的变化。结果大鼠模型建立后,后肢机械缩爪阈值明显降低(P<0.01),其外周血IgG、IgM水平出现有统计学意义的升高(P<0.05),髓核中出现抗原抗体复合物阳性沉积(P<0.01),其相应节段神经根出现较明显的炎性反应。结论腰椎间盘突出症中存在自身体液免疫异常,这可能是其发病机制之一。

Objective To investigate the abnormal autoimmunity in humoral immunity of lumbar disc herniation（LDH） on the animal. Methods An non-mechanical pressure lumbar disc herniation model was established on Sprague Dawley rat. The mechanical withdrawal thresholds of posterior limbs were measured. The immunoglobulin G and immunoglobulin M in peripheral blood were examined by turbidimetry. The presence and distribution of antigen-antibody complexes in nucleus pulposus were examined by immunohistochemical method.And the nerve root were observedby hematoxylin and eosin staining. Results After the model establishment,the mechanical withdrawal thresholds of posterior limbs were significantly reduced （P0.01）,the peripheral IgG and IgM levels were by hematoxylin and eosin staining. Results After the model establishment,the mechanical withdrawal thresholds of posterior limbs were significantly reduced （P0.01）,the peripheral IgG and IgM levels were elevated（P0.05）. And there were positive presence of antigen-antibody complexes in the nucleus pulposus（P0.01）and obvious inflammatory lesion in the nerve root after model establishment. Conclusion There was abnormal autoimmunity in the lumbar disc herniation,which could be one of its pathogenesis.