新鲜人关节软骨试件在体外不同环境下退变的组织学观察

Degeneration of fresh human articular cartilage specimens under different environmental conditions:A histological observation

背景:采用新鲜人软骨试件是进行生物力学实验的最佳选择,但当实验不能及时完成时,何种保存方法能最大限度地保护软骨样本,使其退变达到对实验影响最小是关键。目的:观察新鲜人关节软骨试件在体外不同环境下的组织学变化,寻找试件最佳保存方法和时间。方法:将新鲜人关节软骨试件分为4组:正常对照组,以软骨用固定液固定;空气暴露组,暴露于空气;生理盐水组,以浸湿的生理盐水纱布包裹,滴注生理盐水;林格氏液组,以浸湿的林格氏液纱布包裹,滴注林格氏液。处理后1,2,3h,进行苏木精-伊红染色、Masson三色染色法、番红O染色。结果与结论:染色结果显示,生理盐水或林格氏液滴注1h,关节软骨表面无明显改变,3h后则出现较明显的变化,软骨表面出现明显的粗糙纤维,排列紊乱,并可见较多的裂隙。空气暴露1h软骨表面即已出现皲裂,3h后已失去关节软骨表面的田埂样外观,出现甚大裂隙和纤维暴露,产生软骨损伤。提示以生理盐水或林格氏液纱布包裹新鲜人离体关节软骨试件均是有效的处理方法,并且软骨保存时间最好在离体后2h内。

BACKGROUND：The fresh human cartilage specimens are the best choice for biomechanical experiments,but when the experiment could not be completed in time,what method can save the maximum protection of cartilage samples so that their degeneration to achieve the least impact on the experiment.OBJECTIVE：To observe fresh specimens of human articular cartilage in vitro under different circumstances,and to look for the best specimen preservation methods and time.METHODS：Fresh human articular cartilage specimens were divided into four groups：normal control group,in order to cartilage fixed with the fixative;air-exposed group,exposed to the air;saline group,the saline soaked gauze package and drip of saline;Lin Grignard solution group,with gauze soaked in Ringer＇s solution package,drip of Ringer＇s solution.After treatment for 1,2,and 3 hours,the specimens were performed with hematoxylin-eosin staining,Masson tri-color staining,and Safranin 0 staining.RESULTS AND CONCLUSION：Staining results showed that following saline or Ringer＇s droplet injection for 1 hour,no significant changes were found at articular cartilage surface;3 hours later,rough fibers were observed at surface of cartilage and arranged in disorder,while more cracks were observed.After exposing in the air for 1 hour,rhagades was found at surface of cartilage;3 hours later,ridge-like appearance was unclear,large cracks were observed,and fibers were exposed resulting in cartilage injury.This suggested that tips to saline or Ringer＇s solution gauze wrapped fresh human articular cartilage specimens in vitro were effective,and cartilage preserved preferably within 2 hours after exposure in vitro.