比格犬黑皮质素受体3基因A167T对体质量性状的影响

Effects of melanocortin-3 receptor gene A167T on the body weight trait of Beagle dogs

背景:MC3R基因突变是引起体质量增加的重要因素之一。目的:分析比格犬MC3R基因突变与体质量性状的关系。方法:抽取112只比格犬血液,记录体质量数据,然后提取DNA;采用AS-PCR技术对MC3R基因A167T位点进行分析,并克隆测序。观察比格犬MC3R基因T167A的基因型分布及其与体质量的关系。结果与结论:167位点表现为A、T2个等位基因和AA、AT及TT3种基因型;T167A碱基置换对犬体质量有显著影响(P<0.05)。表明MC3R基因突变可致犬体质量增加,可作为犬体质量标记的候选基因。

BACKGROUND：Melanocortin-3 receptor（MC3R） gene mutation is an important factor in weight gain.OBJECTIVE：To analyze the relationship between MC3R gene mutations and body weight traits in Beagle dogs.METHODS：The blood samples were taken from 112 Beagle dogs,and their body weights were recorded,then their genomic DNA was extracted.The A167T site of MC3R gene was detected by the methods of AS-PCR,and mutant-type MC3R gene was sequenced.The relationships between MC3R genotypes distribution and body weight were observed.RESULTS AND CONCLUSION：The A167T site of Beagle canine MC3R gene was found with two alleles A and T and three genotypes AA,AT and TT.Statistical analysis showed that the base substitution of T167A had significant influence on canine body weight（P〈0.05）.It suggested that MC3R gene mutation can lead to a significant increase in canine body weight,which can be used as a candidate gene for body weight labeling.