胎儿颌面部皮肤透明质酸的含量分析(英文)

Hyaluronic acid content in fetal maxillofacial skin

背景:伤口处细胞外基质中高含量的透明质酸对瘢痕形成有抑制作用,胚胎皮肤切口呈现无瘢痕愈合,但对于胎儿皮肤中透明质酸的含量未见报道。目的:观察胎儿颌面部皮肤中透明质酸含量及其变化趋势,并比较与其他部位的差异。方法:取32例3～8月龄引产胎儿的颌面部、背部、腹部及大腿内侧四处皮肤,按取后液氮速冻,按部位分为不同月龄组。标本称质量后剪碎,加生理盐水制成组织匀浆,取上清液,用放射免疫法测定其中透明质酸含量。结果与结论:3～8月龄胎儿颌面部皮肤中的透明质酸平均质量分数为(303.85±80.28)μg/g,腹部皮肤为(308.09±61.17)μg/g,背部皮肤为(276.38±58.52)μg/g,腿部皮肤为(304.27±60.51)μg/g。胎儿颌面部及背部皮肤中透明质酸含量3,4,5月龄高于6,7,8月龄(P<0.05),腹部皮肤4,5月龄高于6,7,8月龄(P<0.05),腿部皮肤3～6月龄高于7,8月龄(P<0.05)。胎儿颌面部皮肤及其他部位皮肤透明质酸含量均高于成人;各部位皮肤均呈现孕中、后期透明质酸含量下降,但下降时间不一致。

BACKGROUND：High level of hyaluronic acid（HA） in extracellular matrix of wounds has been shown to inhibit scar formation and scarless healing has been observed in fetal wounds.However,there is little information regarding HA content in fetal skin.OBJECTIVE：To determine HA content in maxillofacial skin,as well as the changing tendency,and compare with other regions o skin.METHODS：The fetal skin of the maxillofacial region,back,belly,and inner part of leg were harvested from 32 aborted fetuses ranging from 3 to 8 months.All the samples were frozen immediately by liquid nitrogen and preserved at-80 ℃.Different month groups were designated according to skin regions.The samples were weighed and cut into pieces,then grinded into tissue homogenate.HA content in supernatant liquid of all the samples was detected using radioimmunoassay.RESULTS AND CONCLUSION：In 3-8 month-old fetuses,HA content was（303.85±80.28） μg/g in the maxillofacial skin,（308.09±61.17） μg/g in the belly skin,（276.38±58.52） μg/g in the back skin,and（304.27±60.51） μg/g in the leg skin.HA content in the maxillofacial and back skin was higher in 3-5 month-old fetuses than in 6-8 month-old fetuses（P〈0.05）.HA content in the leg skin was higher in the 3-6 month-old fetuses than in the 7-8 month-old fetuses（P〈0.05）.HA content in all parts of skin was higher in fetuses than in adults.In all parts,there was a decline of HA content in the mid-and late-stages of pregnancy,but the decline time differed.