笛鲷鱼类的线粒体DNA控制区结构及其系统发育分析

Structure of the mitochondrial DNA control region of snapper species and their phylogenetic relationship

采用PCR扩增直接测序的方法获得了11种笛鲷鱼类的线粒体DNA控制区全序列。结合从GenBank中下载的6种笛鲷鱼类的相应序列进行结构分析,可以将笛鲷鱼类的控制区分为终止序列区、中央保守区和保守序列区三个区域,找到了终止相关的序列ETAS以及一系列保守序列(CSB-F,CSB-E,CSB-D和CSB-1,CSB-2,CSB-3),并给出了它们的一般形式。以黄谐鱼为外群,采用NJ法和ME法构建笛鲷鱼类的分子系统树。分子系统发育分析表明,线粒体DNA控制区序列适合于笛鲷鱼类的系统发育分析,而且支持Allen关于笛鲷属的系统形态分类学观点。

Complete sequences of the mitochondrial DNA control region from 11 species of snapper species were amplified by polymerase chain reaction（PCR） and directly sequenced.The structure of the control region was analyzed with six other species of snapper from GenBank.According to the alignment,three domains,the termination associated sequence domain（TAS）,the central conserved domain（CD） and the conserved sequence block domain（CSB）,were identified in the mtDNA control region of snapper species.The extended termination associated sequence（ETAS）,three conserved blocks（CSB-F,CSB-D,CSB-D） in the central conserved sequence block domain and three conserved sequence blocks（CSB1,CSB2,CSB3） in the conserved sequence block domain were successfully identified.With Emmelichthys struhsakeri as outgroup,the phylogenetic trees were constructed using the neighbor-joining（NJ） method and the minimum evolution（ME） method.It can be concluded that the control region can be used for the phylogenetic analysis,and the phylogenetic relationships from mtDNA data are consistent with Allen＇s morphology-based system.