40m空气反复暴露对人体体液免疫的影响

Effects of 40 m repetitive air exposure on humoral immunity in humans

目的探讨40m模拟空气反复潜水对人体体液免疫功能的影响。方法16名健康成年男性，连续3d进行6次40m-35min空气模拟潜水。其中8人在高气压暴露前1d开始，每天口服三联抗氧化剂（维生素C1g／a、维生素E2．5g/d、N．乙酰半胱氨酸8g/d）。分别于暴露前1d以及暴露第1天、第2天、第3天下午出舱后立即抽血分离血清，测定补体、免疫球蛋白以及应激指标。结果模拟潜水后，IgA、IgM和c3均较暴露前增加（19〈0．05）；C4、IgG分别在暴露第1天和第3天时增高（P〈0．05）；促。肾上腺皮质激素在第2天、第3天时增高（P〈0．05）。而皮质醇、肾上腺素、去甲肾上腺素以及多巴胺等应激指标暴露前后无明显差异。服用抗氧化剂不影响上述指标的改变。结论40m模拟空气潜水后部分体液免疫指标升高，这种影响可能并非由应激或氧化应激引起。

Objective To explore effects of 40 m repetitive air dives on humoral immunity in healthy volunteers. Methods Sixteen healthy male volunteers experienced 6-time 40 m-35 rain simulated air dives for 3 consecutive days. Eight of them took trilogy antioxidants （ vitamin C 1 g/d, vitamin E 2. 5 g/d, Naeetylcysteine 8 g/d ） orally 1 day before and during 3 days of exposure. Complements, immunoglobutins and stress indices in the serum were measured 1 day before and on 1 st, 2nd and 3rd days of exposures respectively. Results Significant increases in the values of IgM, IgA, C3 were observed, 1 , 2 and 3 days after exposure （P 〈 0.05 ）, and increases in CA, IgG were noted, 1, 2 days after exposure respectively （ P 〈 0.05 ）. Adrenocorticotrophic hormone increased following 2nd and 3rd days of exposure （ P 〈 0. 05 ）. Increases in eortisol, adrenalin, noradrenaline and dopamine showed no statistical significance. Antioxidants did not significantly affect changes induced by diving when compared with the placebo group. Conclusions Increase in the levels of some humoral immunity indices during or after 40 m of simulated repetitive air dives might not be the result of stresses and oxidative stresses.