定量光导荧光法研究含氟涂膜对离体牛牙釉质再矿化的影响

Effect of a fluoride varnish product on remineralization of bovine enamel by QLF method in vitro

目的:定量光导荧光系统(Quantitative Light-induced Fluorescence,QLF)观察含氟涂膜对牛牙釉质脱矿区再矿化的作用。方法:20个新鲜拔除的牛前牙,于每个标本唇面建立4个2mm×2mm开窗区,浸泡在脱矿液中3d,制备早期釉质龋模型后随机分为4组,分别用含氟涂膜、含氟牙膏、10g/L氟化钠液、去离子水处理,置于再矿化液中,并分别于处理的第4,8,16,32天,采用QLF法检测各组标本的荧光损失量△F(%)和病变面积W.S(mm2),计算△Q(%mm2),并再次处理。结果:用含氟涂料处理的牛牙釉质龋标本,再矿化面积最多、△Q降低最大,且与其他处理组有显著的差异(P<0.05)。结论:含氟涂膜有利于增进离体牛牙釉质脱矿的再矿化作用。

AIM：To examine the effect of a fluoride varnish product on the remineralization of bovine enamel using Quantitative Light-induced Fluorescence （QLF） in vitro. MEHTODS： Twenty extracted bovine anterior teeth were used in this study. On the labial surface of each tooth, 4 artificial subsurface lesion windows （2 mm×2 mm） were created by immersion in demineralizing solution. The windows were randomly divided into 4 groups （n=20/group） and were treated with fluoride varnish, a commercially available toothpaste containing 900 ppm F^-, 1% sodium fluoride solution and deionized water, respectively . The average fluorescence loss F （%） and lesion size （mm^2） were determined with QLF at the 4th, 8th, 16th and 32nd day after treatments. Another variable, delta Q, which was defined as the fluorescence loss integrated over the lesion size （%, mm^2）, was calculated. RESULTS： The results showed that the fluoride varnish significantly （P〈0.05） increased the remineralization size and reduced delta Q values as compared with the other 3 groups. CONCLUSION： These findings suggested that fluoride varnish might be useful to enhance the remineralization in vivo.