丙酮醛对人牙周膜成纤维细胞基因表达影响的实验研究

Methylglyoxal induces gene expression profile changes in human periodontal ligament fibroblasts

目的:利用基因芯片,研究丙酮醛刺激前后人牙周膜成纤维细胞的差异表达基因。方法:组织块培养法获取人牙周膜成纤维细胞,取4～7代的细胞进行实验,以含丙酮醛液及不含丙酮醛液的培养液分别培养细胞24h,利用基因芯片分析丙酮醛刺激人牙周膜成纤维细胞前后差异表达基因。结果:基因芯片分析发现丙酮醛刺激人牙周膜成纤维细胞后有5条上调基因和3条下调基因,其中部分基因与细胞代谢、出血及癌症相关。结论:丙酮醛可影响人牙周膜成纤维细胞某些基因的表达。

AIM：To analyze the differential gene expression in human periodontal ligament fibroblasts （HPDLFs） induced by methylglyoxal （MG） through gene chip technology. METHODS： HPDLFs were isolated from healthy periodontal ligaments of subjects undergoing tooth extraction for orthodontic treatment. For differential gene expression study, HPDLFs were cultured in DMEM with or without designated MG for 24h. The differential gene expression in HPDLFs stimulated by MG was analyzed by gene chip technology. RESULTS： Gene gene expression profile analysis showed that 5 genes were upregulated and 3 genes were downregulated when cells were treated with MG. Further analysis demonstrated that those genes were related to cell metabolism and cancer. CONCLUSION： MG has cytotoxic effects on HPDLFs and alter the gene expression of HPDLFs.