荷叶离褶伞可溶性多糖提取工艺研究

Study on extraction technology of soluble polysaccharides from Lyophyllum decastes Sing

对荷叶离褶伞(Lyophyllum decastes Sing)可溶性多糖的提取工艺进行了研究,通过正交设计和方差分析确定了多糖的最佳提取条件。结果表明:时间和温度对荷叶离褶伞多糖的提取影响较大,其最佳工艺为温度85℃、pH9.0、料液比1∶15、提取时间1.5h,用苯酚-硫酸法测定最佳提取工艺条件下固形物中多糖含量为65.52%。

The effects of temperature,solid-liquid ratio,time and pH on the polysaccharides of Lyophyllum decastes were investigated. The optimum extractive condition of polysaccharides was determined through orthogonal experiment design and variance analysis.The experimental results indicated that the reaction time and temperature significantly affected the extraction percent of the soluble polysaccharides,the optimum extraction conditions were temperature 85℃,pH9.0,solid-liquid ratio 1∶15 and extraction time 1.5h. Under these condition,the extraction percent of soluble polysaccharides was 65.52% in solid.