超临界CO_2萃取废弃绿豆种皮中的绿色素

Extraction of green pigment from mung bean skin by supercritical CO_2

以生产绿豆芽所废弃的绿豆种皮为原料,采用超临界CO2萃取方法,研究了萃取绿豆皮色素的最佳工艺条件;借助紫外-可见光谱、高效液相色谱和荧光光谱,判定绿豆皮色素成分;用所提取的绿豆皮色素制备了叶绿素铜钠,并对其性质进行了检验。研究结果显示:超临界CO2萃取绿豆皮色素的最佳工艺是:萃取压力30MPa、萃取温度40℃、萃取时间80min、CO2流量50kg/h、95%乙醇作为夹带剂;初步判定绿豆皮色素的主要成分是叶绿素;制备的叶绿素铜钠各项质量指标达到或超过QB/T3783-1999标准的要求。

The optimum conditions of extracting green pigment from the skin of mung bean was studied with supercritical CO2. The composition of the green pigment was determined by UV-visible spectrum,HPLC and Fluorescent Spectroscopy. The sodium copper chlorophyllin was prepared by the green pigment of mung bean skin,and its nature was analyzed. The results showed that the obtain optimum extraction conditions were as follows：extraction pressure 30MPa,temperature 40℃,extraction time 80min,the flow of CO2 50kg/h and 95% ethanol as a carrying agent.The initial finding of main color composition of the green pigment was chlorophyll. All the quality standards of prepared sodium copper chlorophyllin met or exceeded the requirements of QB/T3783-1999.