摘要
以豆粕为原料,利用酶水解工艺制备复合L-氨基酸,将氯化镁与复合L-氨基酸螯合制备复合L-氨基酸镁。结果表明:豆粕酶水解最佳工艺条件为:固液比1∶25、pH8.5、加10%胰蛋白酶与8%植物蛋白酶、酶解时间4h,所得豆粕水解度为37.80%。复合氨基酸与氯化镁螯合的最佳工艺条件为:配位摩尔比3∶1、pH8.0、温度80℃、时间50min,此条件下螯合率为45.82%。经红外光谱分析,复合L-氨基酸与氯化镁之间存在螯合作用。
This study aimed at the chelation technology of chlorine mabnesium and complex L-amino acid from soybean meal by enzymatic hydrolysis. The results showed that the optimum enzymatic hydrolysis condition for complex L-amino acid were as follows:material to liquid ratio 1∶25(w/v),pH8.5,hydrolysis time 4h of 10%(addition amount)trypsin and 8%(plant proteolytic enzyme)papain respectively. The hydrolysis rate was 37.80% under these conditions. The optimum chelation conditions for chlorine magnesium and comples L-amino acid were determined through orthogonal test as follows:Chelation molar ratio 3∶1,pH8.0,temperature 80℃,time 50min. The chelation ratio was 45.82% under these conditions. Finally the chelation of chlorine magnesium and comples L-amino acid were confirmed by infrared spectroscopy.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第5期264-266,269,共4页
Science and Technology of Food Industry
基金
江苏省科技发展计划项目(BE2006308)
关键词
豆粕
复合L-氨基酸
氯化镁
螯合
复合L-氨基酸镁
soybean
complex L-amino acid
chlorine magnesium
chelation
complex L-amino acid magnesium