摘要
为测定维生素D3及其它光转化异构体,研究确定了HPLC-ELSD测定的色谱分离条件：KromasilC18柱（4.6×250mm,5μm）,流动相为体积比90∶10甲醇一水溶液,流速1.2mL/min,蒸发光散射检测器漂移管温度为80℃,气体流量1.8L/min。在此条件下,各组分得到有效地分离,色谱峰面积的自然对数与浓度的自然对数呈良好的线性关系（r〉0.997）,精密度RSD为1.25%-2.01%,最低检测限为0.05-0.3μg,回收率测定值为98.3%-101.8%。
An HPLC-ELSD method for the determination of vitamin D3 and its isomers was studied. Chromatographic analysis was carried out on a Kromasil C18 column（4.6mm×250mm,5μm） with methanol-water （90∶10,V/V）as the mobile phase. The flow rate was 1.2mL/min.The drift tube temperature was at 80℃ and the gas flow rate was 1.8L/min.The relative standard deviation（RSD） was below 2.01%（n=6）,the detection limits attainable was in the range of 0.05μg for PreVD3 to 0.3μg for 7-DHC,the recoveries was from 98.3% to 101.8%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第5期351-352,355,共3页
Science and Technology of Food Industry
基金
国家自然科学基金(30660126)
广西南宁市科学技术委员会创新基金[南科(1999)62号]
广西大学科研基金项目(20090046)
