高效液相色谱法检测蜂胶中黄酮类物质的研究

Study on determination of flavones in propolis by HPLC

采用高效液相色谱-二极管阵列检测器测定蜂胶中芦丁和槲皮素的含量。用去离子水浸泡-甲醇回流提取法对蜂胶进行预处理,除去蜂胶中的蜂蜡及脂溶性杂质。色谱柱为EclipseXDB-C18(150mm×4.6mm,5μm),以甲醇和H3PO4缓冲液(V∶V=60∶40)为流动相,流速为0.45mL/min,在370nm处同时测定芦丁、槲皮素的含量。结果表明:芦丁、槲皮素的线性回归方程相关系数R2分别为0.9995和0.9996,回收率分别为96.48%～100.66%;RSD分别为1.25%～4.19%。该方法简便、灵敏、快速、可靠、重现性好,本研究可为蜂胶生物活性评价及质量控制提供科学依据。

HPLC method was applied to determin rutin and quercetin in propolis. Beeswax and fat soluble impurities were removed when deionized water immersion-methanol extraction was used to deal with propolis,The separation was performed on Eclipse XDB-C18（（150mm×4.6mm,5μm）,the mobile phase consisted of alcohol and H3PO4 buffer solution（V∶V=60∶40） as the flow phase,the speed of flow was 0.45mL/min,the detector wavelength was set at 370nm. The result indicated that the liner correlation coefficient of rutin and quercetin was 0.9995 and 0.9996,the recovery was 96.48%～100.66%;RSD respectively was 1.25%～4.19%. The method was simple,sensitive,fast,reliable and repeatable. This study provided a scientific method for quality control and bioactivity evaluation in propolis.