乙烯利刺激对檀香叶中多糖含量影响的初步研究

Study on effect of ethrel stimulation on content of polysaccarides in leaves of Santalum album L.

目的:建立檀香叶水溶性多糖含量的测定方法,分析乙烯利刺激对檀香叶中多糖含量的影响。方法:分别采用蒽酮硫酸法和苯酚硫酸法两种方法,利用紫外-可见分光光度计测定檀香叶中水溶性多糖的含量。结果:两种方法测定的檀香叶中水溶性多糖含量在8.86%～15.61%之间。结论:两种方法简便、准确、重现性好,均可用于檀香叶多糖含量的测定;乙烯利刺激檀香能提高其叶片中水溶性多糖的含量。

Objective： Established a method of determination of the content of polysaccharides in leaves of Santalum album L.,and studied on the effect of ethrel stimulation on content of polysaccarides. Methods： Phenol-Vitriolic colorimetry and anthrone sulphuric acid colorimetry were employed to determine the content of polysaccharides by UV spectrophotography. Results： The content of polysaccharides in leaves of Santalum album L. was between 8.86%～15.61%. Conclusion： The two methods were simple,accurate and with good reproducibility,both of them could be used in the determination of the content of polysaccharides. Ethrel stimulation could raise the content of polysaccharides.