晚期血吸虫病患者肝脾内T细胞亚群的定位观察

OBSERVATION ON THE IOCATION OF T CELL SUBSETS IN THE LIVER AND SPLEEN OF PATIENTS WITH ADVANCED SCHISTOSOMIASIS JAPONICA

采用免疫组织化学技术检测了22例晚期血吸虫病（下称晚血）患者肝内T细胞亚群，发现T细胞集中分布于虫卵肉芽肿外层，在非肉芽肿部位散在分布于虫卵附近，而广泛纤维化的区域内无T细胞浸润。肉芽辟和非内芽肿部位的T细胞均以CD8+细胞为主。19例患者脾脏内T细胞观察，发现脾脏动脉周围淋巴鞘和边缘区的CD4+细胞百分比和CD4+／CD8+比值显著降低，CD8+T细胞百分比明显提高，而且由于脾脏纤维化，导致红素区T细胞稀疏，动脉周围淋巴鞘T细胞外移，本文讨论了以上结果在晚血免疫调控中的作用。

The T cell subsets in the livers of 22 patients with advanced schistosomiasis japonica were detected using immunohistochemical technique. It was found that T cells located concentratively in out-layer of schistosomiasis granuloma. They scattered in non-granuloma zones near insect ova. There was no T cell infiltration in extensive fibrosis zones. CD8+ cells were the main T cell both in granuloma and non-granuloma zones. The spleens of 19 patients were investigated. It showed that CD4+ T cells were located in lymphocyte sheath and marginal zone of spleen artery and their numbers and ratio of CD4+/CD8+ T cells decreased obviously,mean-while the percentage of CD8+ T cell increased obviously. The formation of fibrsis led to the reduction of T cells in red medulla and T cells of lymphocyte sheath located in artery moved outside. The immune regulation of advanced schistosomiasis japonica was discussed.