鲀形目3种鱼的染色体组型分析

Karyotypes Analysis for Three Species of Tetraodontiformes Fishes

采用胸腔注射植物血球凝集素(phytohemagglutinin,PHA)及秋水仙素溶液,取活体头肾细胞经低渗、固定、空气干燥法,分析比较了中华单角鲀(Monacanthus chinensis)、黄鳍东方鲀(Takifuguxanthopterus)、红鳍东方鲀(T.rubripes)的核型。结果表明,3种海水鱼中期染色体均为二倍体,未发现异型性染色体、随体和次缢痕。其核型如下:中华单角鲀的核型为2n=34(34t),臂数:NF=34;黄鳍东方鲀的核型为2n=44(12m+8sm+24t),臂数:NF=64;红鳍东方鲀的核型为2n=44(14m+6sm+24t),臂数:NF=64。中华单角鲀的核型与后两者存在较大差异。同时,将此3种鱼的核型与前人报道的其他鲀形目鱼类核型作了比较。

The karyotypes of three species of Tetraodontiformes fishes were analyzed. The PHA and colchicine were injected in vivo,and kidney cells were collected and treated with lower osmotic pressure and air-drying technique. The results showed that these three marine species had diploid chromosome karyotypes,and all did not contain satellite chromosome,secondary constrictions and sex chromosome. The karyotype formula were as follows： the karyotype of Monacanthus chinensis was 2n = 34,34t,NF = 34; the karyotype of Takifugu xanthopterus was 2n = 44,12m ＋ 8sm ＋ 24t,NF = 64; and the karyotype of T. rubripes was 2n = 44,14m ＋ 6sm ＋ 24t,NF = 64; We also compared the karyotypes of the related fishes in the order of Tetraodontiformes.