心源性猝死患者心肌SOCS-1和SOCS-3表达的研究

Preliminary observation of the expressions of SOCS-1 and SOCS-3 in myocardium of patients with sudden cardiac death

目的 探讨心源性猝死（sudden cardiac death,SCD）患者心肌中细胞因子信号转导抑制分子-1（suppressor of cytokine signaling-1,SOCS-1）和SOCS-3的表达及临床意义.方法 取2005-2006年中山大学法医学系的尸检心肌标本24例,其中9例尸检符合冠状动脉粥样硬化（非心肌梗死）导致SCD（简称非心梗组）,7例尸检符合急性心肌梗死猝死（简称心梗组）,对照组8例选择了因交通事故、外伤等所致死亡的患者.以RT-PCR法检测对照组、非心梗组和心梗组心肌中SOCS-1,SOCS-3的mRNA表达,用免疫组化法检测对照组、非心梗组和心梗组心肌中SOCS-1,SOCS-3的蛋白表达.数据以SPSS13.0统计学软件处理,采用方差分析检验.结果 非心梗组和心梗组患者心肌中SOCS-1 mRNA,SOCS-3 mRNA的表达量均显著高于对照组,分别为[（0.788±0.101）,（0.741±0.111）vs.（0.436±0.044）,P〈0.01],及[（0.841±0.092）,（0.776±0.070）vs.（0.454±0.076）,P〈0.01].非心梗组和心梗组患者心肌中SOCS-1的蛋白抗体阳性细胞数均显著高于对照组,分别为[（320.00±48.48）,（347.14±70.88）vs.（42.50±10.35）,P〈0.01].非心梗组和心梗组患者心肌中SOCS-3的蛋白抗体阳性细胞数均显著高于对照组,分别为[（381.11±59.25）vs.（40.00±10.69）,P〈0.01]和[（332.86±111.91）vs.（40.00±10.69）,P〈0.01].结论 冠心病所致SCD患者的心肌中SOCS-1,SOCS-3的表达显著增加,提示它们可能参与了SCD的发病机制.

Objective To investigate the expressions and clinical significanees of suppressors of cytokine signaling-1 （SOCS-1） and SOCS-3 in myocardium of patients with sudden cardiac death （SCD）. Method This study included myocardial autopsy specimens of 24 patients admitted between 2005 and 2006. Of them, 9 cases had the findings of autopsy examination consistent with coronary atberosclerosis （non-myocardial infarction） leading to SCD （non-MI group）, 7 patients died of acute myocardial infarction （MI group） and 8 patients died of traffic accidents and trauma The expressions of SOCS-1 mRNA and SOCS-3 mRNA in the myocardium of non-MI and con-trol group were detected by using RT-PCR. The levels of SOCS-1 protein and SOCS-3 protein were detected by us-ing immunohistochemistry. Statistical analysis were performed by using SPSS version 13.0 software and the data were processed with ANOVA test. Results The expressions of SOCS-1 mRNA and SOCS-3 mRNA in non-MI and MI groups were were significantly higher than those in control group （0. 788±0. 101） and （0. 741±0.111） vs.（0.436±0.044） （P 〈0.01）; （0.841±0.092） and （0.776±0.070） vs.（0.454±0.076）, P 〈0.01, re-spectively）. The antibody-positive cells of SOCS-1 protein in myocardium of non-MI group and MI group were significantly higher than those in myoeardium of control group （320.00±48.48） and （347.14±70.88） vs.（42.50±10.35） （P 〈 0.01）, respectively. The antibody-positive cells of SOCS-3 protein in myoeardium of non-MI group and MI group were significantly higher than those in myocardium of control group （381.11±59.25） vs.（40.00±10.69）, （P 〈 0.01）and （332.86±111.91） vs. （40.00±10.69）, （P =0.001）. Conclusions The expressions of SOCS rnRNA and SOCS-3 mRNA in myoeardium of patients with SCD from coronary diseases are significantly increased contributing to the pathogenesis of SCD.