COX-2和PCNA在膀胱移行细胞癌中表达的意义及其相关性研究

The significance and correlation of COX-2 and PCNA expressions in bladder transitional cell carcinoma

目的探讨COX-2和PCNA在膀胱移行细胞癌（BTCC）中的表达及临床意义。方法采用免疫组化S-P法，检测61例BTCC和10例正常膀胱组织中COX-2和PCNA的表达。结果COX-2和PCNA在正常膀胱移行细胞中不表达，在BTCC中阳性率分别为75．41％（46／61），70．49％（43／61），两种基因在正常膀胱移行细胞和BTCC中的表达之间差异有统计学意义（P〈0．01）；两种基因的阳性表达水平与BTCC中的病理分级、临床分期有关（P〈0．01）；PCNA在BTCC的表达水平与BTCC的复发率有关（P〈0．05），两种基因在BTCC中的表达水平之间呈正相关（rs=0．546，P〈0．01）。结论COX-2和PCNA异常表达在BTCC发生、发展、转移、复发等过程中起着重要作用。联合检测能更好地判断BTCC的预后和制定合理治疗方案。

Objective To evaluate the expressions of COX-2 and PCNA in bladder transitional cell carcinoma （BTCC） and its prognostic significance. Method The expressions of COX-2 and PCNA were detected by S-P immunohistochemical technique in 61 cases of BTCC and 10 cases of normal bladder tissues. Results No positive expressions of COX-2 and PCNA were found in normal bladder tissues, and it was significantly different between the normal bladder tissues and BTCC （ P 〈 0. 01 ）. The expression level of COX-2 and PCNA elevated as the grade and stage advanced in BTCC. COX-2 level in BTCC was positively related with PCNA level. Conclusions The abnormal expressions of COX-2 and PCNA play an important role in the development of BTCC. COX-2 and PCNA are good molecular markers of BTCC, and it is helpful for the assistant diagnosis, prediction of prognosis and reasonable therapeutic regimen.