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BRCA1启动子甲基化与肝癌的关系研究

Relationship of BRCA1 promoter methylation and hepatocellular carcinoma
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摘要 目的探讨BRCA1基因启动子区甲基化与肝癌发生的关系。方法采用甲基化特异性PCR检测肝癌细胞系和肝癌组织标本的甲基化状态。结果5个肝癌细胞系中2个(PLC-PRF-5和97H)发现甲基化,3个未发现甲基化;在39例肝癌组织标本中22例发生甲基化(56.4%)。结论BRCA1启动子甲基化与肝癌的发生有关,可能作为肝癌的诊断标志物。 Objective To explore relationship of BRCA1 promoter methylation and hepatocellular carcinoma.Methods BRCA1 promoter methylation in hepatocellular cancer cell lines and cancer tissues were detected by methylation specific PCR.Results Two of five hepatic cancer Cell lines（PLC-PRF-5 and 97H） were methylated and 22 hepatic cancer tissue samples were methylated（56.4%）.Conclusion BRCA1 is frequently methylated in hepatocellular carcinoma.It may be a hepatocellular carcinoma biomarker.
作者 张晓梅 杨云生 郭明洲
机构地区 中国人民解放军总医院消化科
出处 《胃肠病学和肝病学杂志》 CAS 2010年第4期317-318,共2页 Chinese Journal of Gastroenterology and Hepatology
关键词 肝细胞癌 BRCA1 DNA甲基化 甲基化特异性PCR Hepatocellular carcinoma BRCA1 DNA methylation Methylation specific PCR
分类号 R730.23 [医药卫生—肿瘤]
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参考文献10

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胃肠病学和肝病学杂志
2010年 第4期

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