经裸鼠体内多次传代所致的高致瘤HL-60细胞模型的建立及其细胞生物学特征

Establishment and Characterization of Highly Tumorigenic Leukemia Cell Line HL-60 Transplanted through Repeated Passages into Nude Mice

本研究探讨裸鼠体内多次传代的高致瘤性HL-60细胞的生物特性的变化,探索高致瘤性HL-60细胞致瘤率提高的机制。用裸鼠体内和体外传代方法建立高致瘤性白血病细胞系HL-60模型,并对其生物特性改变进行比较。用台盼蓝染色法检测HL-60细胞生长水平,流式细胞术分析细胞周期,电子显微镜观察HL-60细胞的超微结构和荧光水平。结果表明:细胞生长速度加快,细胞群体倍增时间缩短;S期细胞比率有上升的超势。细胞线粒体数目显著下降(p<0.01),且多数内室扩张,嵴数目减少,有的空泡化;细胞微丝荧光减弱,差异显著(p<0.01);克隆形成率有明显的增加。结论:裸鼠体内多次传代的HL-60细胞高致瘤性与致瘤细胞增殖能力增强,细胞中线粒体数目与结构的改变和细胞骨架微丝的变化有关。

This study was purposed to investigate the change of biological characteristics of HL-60 cells with high tu- morigenicity transplanted through repeated passages into nude mice and to explore the tumorigenic mechanisms of the cell line. The human highly tumorigenic leukemia cell line HL-60 model in nude mice were established by serial passages in vivo and in vitro, and their biological features were compared. The trypan blue staining assay was used to detect the cell growth, the flow cytometry was used to analyze the cell cycle, the transmission electron microscopy and laser scanning confocal microscopy were used to observe the cell ultrastructures and cell fluorescence level respectively. The results in- dicated that the cell growth velocity was quickened, cell doubling time was shortened in high tumorigenic leukemia cell line HL-60; the cell count in S phase increased; the amount of mitochondria in HL-60 cells obviously decreased, further- more the dilation of interspace, decrease of the number of ridges, vacuolation of mitochondria, significant reduction of fluorescence level in microfilament and enhancement of cell cloning efficacy were observed. It is concluded that the high tumorigenicity of HL-60 cells multiple-passaged in nude mice is associated with enhancement of proliferative ability, changes of number and structure of mitochondria in HL-60 cells, and alteration of microfilament in cytoskeleton.