25Gy γ射线辐照对手工富集人血小板CD62p表达的影响

Effects of 25 Gy γ-ray Irradiation on the Expression of CD62p in Manually Enriched Human Platelets

本研究观察25Gyγ射线照射对22℃保存条件下的手工富集血小板悬液不同保存期CD62p、血小板计数及平均血小板体积(MPV)的影响。将富浆法分离的16袋血小板,每袋平均分为两份,其中1袋经25Gy137铯γ射线辐照,另1份不辐照,然后按美国血库协会(AABB)标准保存72小时。经辐照的血小板作为观察组,未辐照的血小板作为对照组。流式细胞术检测两组血小板辐照前及保存24和72小时后P选择蛋白的表达水平;同时用血细胞计数仪检测血小板数和平均血小板体积(MPV)。结果表明:辐照组与对照组血小板表达CD62p百分率均随保存时间的延长而增高;保存24小时与新鲜血小板比较有显著性差异(p(0.05),保存72小时与保存24小时比较有非常显著性差异(p(0.01)。在保存24、72小时后,两组血小板CD62p表达率、血小板计数无显著差异(p>0.05);但两组血小板在保存72小时后MPV与新鲜血小板比较有显著性差异(p(0.05)。结论:γ射线照射不影响血小板的数量和质量,但手工血小板液态储存时间应尽可能缩短。

This study was purposed to investigate the effects of 25 Gy -y-ray irradiation on the CD62p expression, platelet count and the mean platelet volume （MPV） of manually enriched platelet suspension in different time of shelf life at 22℃. Each of 16 bags with plasma-rich platelet was divided into two bags, one of which was exposed to 25 Gy -y-ray of 37Cs and the other ones was not exposed. 16 bags then were preserved for 72 hours according to AABB standards. The irradiated platelets were regarded as the observation group, and the other ones were regarded as the control group, the expression of p-selectin （CD62p） in the above 2 groups was detected by flow cytometry before irradiation and at 24, 72 hours after irradiation respectively; at the same time, the platelet count and MPV were assayed by using blood cell counter. The results showed that the expression level of CD62p on platelet in irradiated and control groups increased along with the prolonging of preservation time, the expression rate of CD62p on the platelets preserved for 24 hours was higher than that on fresh platelets with significant difference （p 〈 0.05 ） ; the expression rate of CD62p on the platelets preserved for 72 hours obviously was enhanced as compared with platelets preserved for 24 hours （p 〈 0.01 ）. There were no significant differences in CD62p expression rate, platelet count and MPV between irradiated and control groups preserved for 24 and 72 hours （p 〉0.05）, however the MPV of irradiated and control groups preserved for 72 hours was higher than that of fresh platelets （p 〈 0.05）. It is concluded that the γ-ray irradiation does not affect the quantity and quality of platelets, but the preservation time for manually enriched platelet suspension should be shortened as far as possible.