农杆菌介导获得高亲和NH_4^+转运因子转基因甜菜

Transgenic Sugar Beet Harbouring High-affinity NH_4^+ Transporter Gene Acquired by Agrobacterium-mediated Transformation

本研究利用农杆菌介导的方法将高亲和NH4+转运因子融合基因转入甜菜子叶节外植体。遗传转化过程主要包括子叶节预培养、侵染转化、共培养、洗菌、抑菌培养、潮霉素筛选分化培养、抗性苗生根移栽。实验获得抗性苗140株,抗性芽分化率为8.14%,随机抽取24株抗性苗进行PCR检测,获得转基因植株10株。

In this study, high-affinity NH4＋ transporter fusion gene was successfully transformed into the cotyledon node explants of sugar beet by using Agrobacterium-mediated transformation methord. Genetic transformation process includes cotyledon node pre-culture, infection transformation, co-culture, washed bacteria, bacteriostatic culture, differentiation culture of hygromycin selection, and resistance seedlings to root transplanting. As the experimental result, 140 resistant seedlings were obtained and its differentiation rate was 8.14%, 24 resistant seedlings were selected randomly to PCR detecte, and PCR assaying of plant DNA confirmed 10 transgenic plants.