黑芥与花椰菜体细胞杂种不同世代遗传变异的SRAP分析

Genetic Variation of the Different Offsprings Derived from Somatic Hybrids of Cauliflower and Black Mustard Revealed by SRAP

本文利用SRAP标记对黑芥与花椰菜体细胞杂种回交一代到回交三代(BC1、BC2、BC3)及自交二代到自交四代(S2、S3、S4)的遗传变异进行了分析。20个引物组合共产生528条扩增带,其中多态性条带168条,平均每组引物产生8.4条多态性标记。结果表明,杂种后代各世代间都存在着不同程度的遗传变异,但变异的程度较小于预期值。在聚类图中,自交后代和回交后代被明显的区分成为两个类群。自交后代与黑芥的遗传距离较近,回交后代与花椰菜的遗传距离较近;自交后代的不同世代间变异程度要小于回交后代,但各世代间遗传距离的差异性并不显著(P>0.05)。

In this research, we analyzed the genetic variability of three selfing offsprings （S2, S3 and S4） and backcross offsprings （BC1, BC2 and BC3） of cauliflower-black mustard somatic hybrids by using sequence-related amplified polymorphism （SRAP） markers. A total of 528 bands were generated with 20 primer pairs, including 168 polymorphic bands with an average of 8.4 per primer combination. The results showed that the different offsprings of cauliflower-black mustard somatic hybrids had various degree of genetic variation, however, The extent of that was less than we expected. The offsprings of cauliflower-black mustard somatic hybrids were divided obviously into two parts of selfing offsprings and backcross offsprings in the cluster tree. The selfing offsprings had closer genetic distance to black mustard, The backcross offsprings had closer genetic distance to cauliflower. The hereditary variation degree of the selfing offsprings were less than that of backcross offsprings. But the offsprings of cauliflower-black mustard somatic hybrids had no significant difference in genetic distance （P0.05）.