结球甘蓝迟抽薹基因RAPD标记转SCAR标记

A SCAR Marker Derived from the RAPD Marker Linked to Later Bolting Gene in Headed Cabbage

本研究以与结球甘蓝迟抽薹基因连锁的N1750为引物,应用RAPD技术进行PCR扩增,检测到迟抽薹基因,对特异片段进行回收、克隆和测序,依据测序结果设计SCAR引物。在166株BC1群体(A21与P02杂交得到F1再与P02回交)中通过与RAPD标记的比较,SCAR扩增结果同RAPD扩增结果完全一致,从而证实了SCAR标记的准确性。实验结果表明,与甘蓝迟抽薹基因连锁的RAPD标记被成功转化为SCAR标记,为甘蓝分子标记辅助选择育种提供了基础。

In this study, we amplified later bolting gene by using N1750 as primer linked to later bolting gene of headed cabbage and RAPD technique as approach, and then recovered, coloned and sequenced the specific fragments. A pair of specific SCAR primers was designed based on the obtained sequence. SCAR amplification result was identical with that by RAPD markers in 166 BC1 individuals derived from F1 （A21×P02） and P02 （as recurrent parent）, which verified the accuracy of SCAR markers. The results demonstrated that the RAPD marker was converted to SCAR marker successfully, which provided a basis for marker assisted breeding in headed cabbage.