茶树离体植株再生与遗传转化

Plant Regeneration and Genetic Transformation in Camellia sinensis

茶树(Camellia sinensis(L.)O.Kuntze)属山茶科(Theaceae),是重要的饮料作物。茶树为多年生木本植物,其自身的一些特性,如长生育周期、自交不亲和性、高度自交衰退等,使人工杂交或自交进行的遗传改良受到诸多限制。因此通过遗传转化进行茶树育种越来越受到研究者的重视。离体再生困难和转化效率低是限制茶树遗传转化进行的主要因素。本文在综述了茶树微繁途径、器官发生途径和体细胞胚发生途径等离体植株再生和遗传转化的主要研究进展基础上,提出发根农杆菌(Agrobacterium rhizogenes)介导法最有可能在茶树遗传转化上取得成功。

Tea plant （Camellia sinensis （L.） O. Kuntze） is one of the important crops for beverage, which belongs to the family Theaceae. Tea plant is one perennial woody plant, there are some characteristics which limit the genetic improvement by artificial interspecific hybridization or selfing of tea, such as long life cycle, self-incompatibility and high inbreeding depression, etc.. The genetic transformation in tea plant breeding becomes more and more attractive. The major limited factors of genetic transformation in tea plant are the difficult plant regeneration and low transformation efficiency. The advance advances in plant regeneration include micropropagation, organogenesis and embryogenesis, and genetic transformation in Camellia sinensis were reviewed, the author suggested that Agrobacterium rhizogenes mediated-transformation maybe a possible successful method in tea plant.