伏曲酵母菌的分类鉴定及来源分析的研究

Study of Classification and Identification to Yeast Strains and the Source

目的:统计分析酵母菌的来源,为大曲微生物物种及其来源变化提供基础资料。方法:利用Biolog微生物自动分析系统进行精确鉴定,并通过统计分析得出微生物菌种的来源。结果:从伏曲样品中分离纯化出7株酵母菌J-1,2,3,4,5,6,7,利用Biolog微生物自动鉴定系统进行鉴定,确定其分别为白吉利丝孢酵母A、无名假丝酵母菌、汉逊德巴利酵母C、海隐球酵母菌、东方伊萨酵母菌、解脂耶罗威亚酵母菌、皱落假丝酵母菌。这些物种主要来源于原料本身和空气,原料为了入室大曲提供了J-1,2,3三种酵母菌,其比例为25.45%、19.23%和7.5%。结论:空气中含有大曲中所有的酵母菌的种类,曲房空气中的酵母菌数量和种类均较多。

Objective：The sources of yeast were abtained by statistic and analysis,which provided basic data of Duqu microorganism species and their sources and changes.Method：Biolog automated microbes identification system（Biolog System）was used to identificated,and the sources of microorganism species were obtained by statistic and analysis.Result：Biolog automated microbes identification system（Biolog System）was used to check seven yeast strains J-1,2,3,4,5,6,7,which were separated and purified from fermented grains of summer Daqu.They were identified as Trichospor beigelii A;Candida famata,Cryptococcus marinus,Debaryomyces hansenii C,Issatchenkia orientalis,Yarrowia lipolytica,Candida rugosa B.These species mainly come from raw material and air,raw material provided J-1,2,3 three yeasts to Daqu,and which ratio were 25.45%,19.23% and 7.5%.Conclusion：Air contains all the types of Daqu yeast,the number and species of yeast were more in Qu-room.