焦化废水中4株苯酚高效降解菌的分离及鉴定

Isolation and Identification of Four Highly Effective Phenol-degrading Strains from Coking Wastewater

目的:从焦化废水中筛选苯酚高效降解菌并进行鉴定。方法:在100～1000mg/L的苯酚为惟一碳源的无机盐培养基上分离出单菌落,测定各菌株的生长曲线以及对苯酚的降解效率;利用16SrDNA序列分析结合菌株的形态特征确定各菌株的分类地位。结果:筛选获得4株苯酚降解菌,均能够以苯酚为惟一碳源,在30℃、pH7.0、摇床转速130r/min、2%的接种量条件下,24h内能将1000mg/L的苯酚降解91%以上;4株菌可初步鉴定为芽孢杆菌属(ZL1)、产碱杆菌属(ZL2、ZL4)、沙雷氏菌属(ZL3)。其中,从焦化废水中分离出高效降解苯酚的沙雷氏菌未见报道。结论:从焦化废水中获得4株苯酚高效降解细菌,对高浓度含酚废水的生物降解具有潜在的应用前景。

Objective：To isolate and identify highly efficient phenol-degrading strain from coking wastewater.Method：The microbes were isolated by the inorganic salt medium containing 100-1 000mg/L phenol as a sole carbon source in order to obtain efficient phenol-degrading strain.The growth curve and phenol-degrading efficiency was determined,also the taxonomic statuses of these strains were determined based on morphological features and 16S rDNA sequences analysis.Result：Four highly effective phenol-degrading bacterial strain named ZL1,ZL2,ZL3,ZL4 were isolated and identified,while strain ZL1 belongs to Bacillus cereus,ZL2 and ZL4 belongs to Alcaligenes faecalis,ZL3 belongs to Serratia sp.The four bacterial strain could utilize phenol as a sole carbon,tolerate phenol of 1 000mg/L,and degrade phenol of 1 000mg/L above 91% in 24h when growing on the inorganic salt medium at temperature 30℃,pH 7.0 and 2% inoculation amount.Conclusion：Four highly effective phenol-degrading bacterial strain were obtained and have potential application in the biodegradation of high concentration organic wastewater with phenol substances.