草麻黄植株再生体系的建立

Establishment of Regeneration System of Ephedrae sinica Stapf

目的:以草麻黄的子叶为材料,建立草麻黄植株再生体系。方法:采用组织培养的方法进行了愈伤组织诱导、愈伤组织分化和不定芽生根研究。结果:MS+2,4-D2.0mg/l+6-BA1.0mg/l为诱导子叶形成具有分化能力愈伤组织的理想培养基;MS+2,4-D1.5mg/l+6-BA1.5mg/l是愈伤组织的最佳继代培养基;MS+IAA0.2mg/l+TDZ2.0mg/l是愈伤组织不定芽分化的最佳培养基,分化率为75%;试管苗生根培养基为MS+2,4-D1.0mg/l。结论:建立了草麻黄子叶再生系统,为开发和保护麻黄野生资源提供一定的材料来源和技术方法。

Objective：To establish the regeneration system of Ephedrae sinica Stapf cotyledon.Method：The conditions needed in callus induction,differentiation and adventitious bud rooting of Ephedrae sinica Stapf cotyledon have been studied.Result：The results showed that the best medium for callus induction was MS＋2,4-D 2.0mg/l＋6-BA 1.0mg/l with ability of differentiation.The best media of callus generation was MS＋2,4-D 1.5mg/l＋6-BA 1.5mg/l.The best media of differentiation was MS＋IAA 0.2mg/l＋TDZ 2.0mg/l,differentiation rate is 75%;The media of root generation was MS＋2,4-D 1.0mg/l.Conclusion：The regeneration system of Ephedrae sinica Stapf cotyledon was established.This test provided material source and technique method for protecting of natural resources of Herba Ephedrae.