摘要
根据GenBank中登录的绵羊朊蛋白编码基因PRNP序列,设计并合成了可用于单核苷酸多态性(SNP)快速分析的3对引物和7条分别标记了FAM和VIC荧光染料的MGB探针,建立了一种利用荧光定量PCR扩增反应对羊痒病抗性基因进行筛选的方法。结果表明,设计的引物及探针具有特异性和高效扩增性,能够用于PRNP羊痒病抗性基因的快速分型。借助该方法可建立一整套完善的预警监测体系来预防该病的发生,也可用于羊痒病的常规实验室诊断。
Three pairs of primers and 7 strips of MGB TaqMan probes marked with FAM and VIC were respectively designed according to the sequences of prion protein genes in GenBank,and used for analysis of single nucleotide polymorphism after real-time PCR amplification.In result,the developed method was specific and high-efficient,and could be used genotype scrapie-resistant genes.The results showed that the developed method,an early-warning and monitoring system would be established to prevent the outbreak of scrapie and the developed method could be used for routine laboratory diagnosis of scrapie,too.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2010年第3期236-239,共4页
Chinese Veterinary Science
基金
国家质检总局科技计划项目(2007IK019)
