线粒体复合物与氧化应激在锰致鸡胚神经细胞凋亡中的作用

Roles of mitochondria complex and oxidative stress in the apoptosis of chicken embryonic neurons induced by manganese chloride

为探讨线粒体复合物及氧化应激在锰致体外培养鸡胚脑神经细胞凋亡中的作用,以体外培养的鸡胚脑神经元为研究对象,在含终浓度为0、1.5、2.0、2.5mmol/LMnCl2的DMEM培养液中培养24h,检测神经元内ROS和GSH含量、线粒体呼吸链复合物活性、细胞凋亡指数。结果显示,随着MnCl2浓度的增加,线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ均呈降低趋势,线粒体呼吸链复合物Ⅴ先升高后降低,细胞内ROS含量增加,GSH含量呈降低趋势,鸡胚脑神经元细胞凋亡指数增加,出现凋亡的形态学变化。表明MnCl2能抑制线粒体复合物活性,引起氧化应激,诱导鸡胚脑神经元发生凋亡。

To explore the roles of mitochondria complex and oxidative stress in the apoptosis of chicken embryonic neurons exposed to MnCl2,the content of ROS and GSH,the activity of mitochondria respiratory chain complex and apoptosis index in neurons cultivated in DMEM with the final concentration of MnCl2 （0,1.5,2.0,2.5mmol/L） for 24h were detected by using the kits.In result,with the increase of the concentration of MnCl2,the activities of mitochondria respiratory chain complexes Ⅰ,Ⅱ,Ⅲ and Ⅳ and the content of GSH decreased.But in 1.5mmol/L MnCl2 environment,the activity of mitochondria respiratory chain complexⅤ decreased,the content of ROS and the apoptosis index increased,and morphological changes of apoptosis were observed in the neurons.These results indicated that MnCl2 could induce the apoptosis of the chicken embryonic neurons which was performed through oxidative stress caused by decreasing the activities of mitochondria respiratory chain complexes.