摘要
目的探讨基质金属蛋白酶-24(MMP-24)基因在卵巢癌细胞株中的表达及临床意义。方法应用半定量RT-PCR和Western blot对卵巢浆液性囊腺癌细胞株OVCa-R3、SKOV3、HO-8910及人卵巢细胞株TC-1进行检测。结果3种卵巢癌细胞株中MMP-24基因和蛋白的表达均增强,与正常卵巢细胞相比,差异有统计学意义。结论MMP-24基因可能与卵巢癌的发生、发展有关。
Objective To investigate the expression of matrix metralloproteinase-24 （MMP-24） gene and MMP-24 protein in ovarian serous cystadenocarcinoma cell lines.Methods The expression of MMP-24 gene and MMP-24 protein of human ovarian serous cystadenocarcinoma cell lines HO-8910、OVCa-R3、SKOV3 were analyzed with RT-PCR and Western blot respectivly.Results High levels of MMP-24 gene and MMP-24 protein were observed in ovarian carcinoma cell lines HO-8910、OVCa-R3、SKOV3,which were significantly higher than those in normal ovarian cells.Conclusion The MMP-24 gene possibly have an important association with the occurrence and development in ovarian carcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第4期411-413,共3页
Cancer Research on Prevention and Treatment
