甲状腺乳头状癌VEGF、MMP-9及COX-2蛋白表达与淋巴道转移和血管生成的相关性

Relationship between Expressions of VEGF,MMP-9,COX-2 and Lymph Node Metastasis and Angiogenesis in Papillary Thyroid Carcinoma

目的通过检测血管内皮细胞生长因子(VEGF)、基质金属蛋白酶-9(MMP-9)、环氧化酶-2(COX-2)在甲状腺乳头状癌(PTC)细胞中的表达情况,探讨它们与PTC颈淋巴结转移的关系。方法采用免疫组织化学SP法检测74例PTC(有淋巴结转移者39例,无淋巴结转移者35例)中VEGF、MMP-9、COX-2的表达,并对CD34表达阳性血管进行MVD计数。结果VEGF、MMP-9、COX-2的表达与MVD值在淋巴结转移组与无转移组之间的差异均具有统计学意义(P<0.05),与PTC淋巴结转移呈正相关;VEGF、MMP-9、COX-2的表达与MVD值正相关(P<0.05),VGEF、MMP-9阳性表达率与肿瘤大小密切相关(P<0.05)。结论甲状腺乳头状癌VEGF、MMP-9、COX-2蛋白表达与其淋巴道转移和MVD有关,检测这几种蛋白表达将有助于判断甲状腺乳头状癌的转移潜能、血管生成能力及预后。

Objective To explore the expressions of vascular endothelial cell growth factor （VEGF）,cyclooxygenase-2 （COX-2）,matrix metalloproteinase（MMP-9） in papillary thyroid carcinoma tissue and their associations with lymph node metastases. Methods The expressions of VEGF,MMP-9,COX-2 and CD34 were examined in 74 cases of papillary thyroid carcinoma including 39 cases with lymph node metastasis and 35 cases without lymph node metastasis with immunohistochemistry. Results The expressions of VEGF,MMP-9,COX-2 and MVD in lymph node metastasis group were significantly higher than those in no lymph node metastasis group （P〈0.05）, and their expressions were positively correlated with the lymph node metastasis of PTC; the expressions of VEGF,MMP-9,COX-2 were correlated with the average of MVD （P〈0.05）; the expressions of VEGF,MMP-9 correlated with size of tumor （P〈0.05）. Conclusion The expressions of VEGF,MMP-9 and COX-2 are strongly associated with lymph node metastasis and MVD of papillary thyroid carcinoma. These proteins are potential indicators of metastasis and predictors for prognosis of PTC.