Survivin反义寡核苷酸转染对肝门部胆管癌细胞FRH-0201侵袭性的影响

Effect of survivin antisense oligodeoxynucleotide on invasive ability of hilar cholangiocarcinoma cell FRH-0201

目的:观察Survivin反义寡核苷酸转染对肝门部胆管癌细胞FRH-0201侵袭能力的影响。方法:针对Survivin基因序列设计合成反义寡核苷酸,阳离子脂质体包裹转染胆管癌细胞株FRH-0201。观察转染后癌细胞生长增殖情况,酶联免疫吸附试验检测细胞上清液中基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶抑制因子-2(TIMP-2)含量,侵袭小室检测细胞侵袭能力的改变。结果:反义转染组细胞生长及增殖减缓并趋向凋亡,细胞上清液中MMP-2因子较正义转染组及阴性对照组少,且差异有统计学意义(P<0.05),TIMP-2因子较另2组有所增加,且差异有统计学意义(P<0.05),Transwell实验中反义转染组穿过小室细胞数目较另2组减少(P<0.05)。结论:Survivin反义寡核苷酸转染可有效抑制肝门部胆管癌细胞增殖,并降低癌细胞的侵袭能力。

Objective： To study the inhibition effect of survivin antisense oligodeoxynucleotide （ASODN）on apoptosis and invasion of hilar cholangiocarcinoma cell line FRH-0201. Methods： ASODN was transfected into cholangiocarcinoma cell line FRH-0201 by LipofectamineTM2000. The level of MMP-2 and TIMP-2 in the supernatant was detected by enzyme linked immunosorbent assay （ELISA）. The effect of ASODN transfection was observed by Transwell cell culture chamber. Results： The cell proliferation was inhibited in the ASODN group. The expression of MMP-2 in ASODN group was lower than those in the SODN and blank group （P0.05）, the level of the TIMP-2 in the ASODN group was higher than those in the other two groups （P0.05）. The cell number through the membrane of the cell culture chamber was decreased in ASODN group than in the other two groups（P0.05）. Conclusion： The ASODN transfection can significantly inhibit the cell proliferative and invasive ability.