PTEN表达下降对乳腺癌细胞生物学能力的影响

Influence of PTEN decent on biological ability of breast cancer cell

目的:探讨PTEN基因在乳腺癌细胞中的表达及对其生物学特性的影响。方法:采用脂质体介导法将PTEN-shRNA转入表达PTEN的M231乳腺癌细胞中,构建PTEN基因沉默的细胞株M231-1548和M231-3001,检测PTEN基因沉默后M231乳腺癌细胞株黏附、转移及增殖能力的改变。结果:转染后M231-1548和M231-3001乳腺癌细胞PTEN蛋白表达量明显减少,其黏附能力明显下降,而迁移、侵袭能力及在三维培养中形成克隆的能力增强。结论:PTEN基因可以促进细胞黏附,抑制细胞增生、迁移和侵袭,对癌细胞具有抑制作用。PTEN基因的缺失可以作为评价乳腺癌患者的进展、转移等预后的指标之一。

Objective： To research the expression of PTEN and its influence on biological ability in breast cancer cell in vivo. Methods： PTEN-shRNA plasmid was transtected into M231 breast cancer cells to knock down the expression of PTEN. The changes of PTEN expression, proliferation, adhesion and metastasis of PTEN knocked down cell were tested by western-blot, colony formation, adhesion and invasion assay. Results： PTEN-shRNA was successfully transfected into M231 cells and it inhibited PTEN expression efficiently.The capabilities of colony formation, migration and invasion of transfected cell were much greater than those of the controlling cell line. But the transfected cells were more difficulty in adhesion than the scrambled ones. Conclusion： PTEN genecan enhance the adhesion, but restrict the proliferation, migration of breast cancer cells in some degree, so that inhibit the development of the breast cancer. PTEN loss can be a prognostic factors for the patients with breast cancer.