犬压力性尿失禁模型的建立

Establishment of animal model for stress urinary incontinence with female beagles

目的建立一种雌犬压力性尿失禁（SUI）模型。方法将9条雌犬随机分为2组，A组4条离断耻骨尿道韧带、切除尿道腹侧及左右侧的纤维结缔组织和脂肪组织，如未出现尿失禁，最终将尿道左右侧、腹侧以及尿道阴道间组织全部切除；B组5条切除尿道阴道间及尿道左右侧的纤维结缔组织和脂肪组织，如未出现尿失禁，再次手术切除尿道左右侧及尿道阴道间因粘连形成的瘢痕组织。结果A组中3条出现尿失禁，B组全部出现尿失禁。结论通过切除尿道周围支持组织来建立雌犬压力性尿失禁模型可行，有成功率高、可直接观察等优点。阴道及尿道阴道间组织在尿控上的作用可能比尿道腹侧组织更大。

Objective To explore the feasibility of eliminating the supportive tissue around the urethra to establish animal model for stress urinary incontinence （SUI） with female beagles. Methods A total of 9 1-year-old female beagles were divided into 2 groups randomly. Group A comprised 4 dogs whose bilateral and ventro-side supportive tissues of urethra were eliminatd and pubourethral ligaments were cut off. If SUI was not observed, the supportive tissue around the urethra was eliminatd ultimately. Group B comprised 5 dogs whose bilateral supportive tissues of urethra and supportive tissue between urethra and vagina were eliminatd. If SUI was not observed, the bilateral adhesive tissues of urethra and adhesive tissues between urethra and vagina were eliminatd. Results Three beagles in group A and all beagles in group B had SUI. The maximum urethral closure pressure of normal and incontinent beagles was （47. 0 ± 15. 6） cm H2O and （28. 6 ± 11. 9） cm H2O, the abdominal leak point pressure of normal and incontinent beagles was （ 131.9 ± 24. 1 ） cm H2O and （74. 0 ± 10. 6） cm H2O, respectively. Conclusion The establishment of animal model for SUI by eliminating the supportive tissue around the urethra of female beagles is feasible, and this model can be observed directly and has high achievement ratio. The vagina and tissue between urethra and vagina may have a greater value in urinary continence than urethral ventro-side tissue （ including pubourethral ligament） in female beagles.