嗜冷杆菌(Psychrobacter sp.)微生物传感器毒性分析性能研究

Toxicity analysis based on a Psychrobacter sp. microbial biosensor

采用嗜冷杆菌(Psychrobacter sp.)微生物传感器毒性分析系统,对Hg2+、Cu2+、Zn2+、Cr6+、Cd2+、Pb2+、Co2+等重金属和邻氯苯酚(2-CP)、2,4-二氯酚(2,4-DCP)、邻硝基酚(2-NP)、对硝基酚(4-NP)、四环素、十二烷基苯磺酸钠(LAS)等有机物的生物急性毒性进行分析.试验结果表明,基于对数生长后期和稳定期的Psychrobacter sp.微生物传感器具有良好的毒性分析性能,呼吸基质盐度低于10%和pH=5～10时,对Psychrobacter sp.微生物传感器的分析性能影响不明显.测试得到Cd2+、Zn2+、Cr6+、Cu2+、Hg2+和Pb2+对Psychrobacter sp.的半数有效浓度(EC50)分别为47.3、10.9、14.0、2.6、0.8和100.1mg·L-1,Co2+的30%有效浓度(EC30)为56.6mg·L-1;4-NP、2,4-DCP、四环素和LAS的EC50分别为424.4、163.8、103.5和754.6mg·L-1,2-NP和2-CP的EC30值分别为249.23和195.6mg·L-1.

Acute toxicities of heavy metals （Hg2＋,Cu2＋,Zn2＋,Cr6＋,Cd2＋,Pb2＋ and Co2＋） and organic compounds （2-CP,2,4-DCP,2-NP,4-NP,tetracycline and sodium lauryl benzenesulfate （LAS）） were determined with a Psychrobacter sp. microbial biosensor. The results showed that when Psychrobacter sp. was in logarithmic and stationary phases,the microbial biosensor showed good performance for acute toxicity analysis. When the substrate salinity （NaCl） was lower than 10% and pH was in the range of 5~10,there was no significant effect on the performance of microbial biosensor. The EC50 values （mg·L-1） to Psychrobacter sp. were Hg2＋ 47.3,Cu2＋ 10.9,Zn2＋ 14.0,Cr6＋ 2.6,Cd2＋ 0.8,Pb2＋ 100.1,Co2＋ 56.6 （EC30）,4-NP 424.4,2,4-DCP 163.8,tetracycline 103.5 and LAS 754.6 mg·L-1,and the EC30 values of 2-NP and 2-CP were 249.23 and 195.6 mg·L-1,respectively.