空间环境诱变甜椒基因组DNA变异的分子检测

MOLECULAR DETECTION OF GENOMIC DNA VARIATION INDUCED BY SPACE ENVIRONMENT IN SWEET PEPPER (Capsicum annuum L.)

为明确空间环境对甜椒后代的诱变影响,本研究以空间诱变的甜椒第4代及其地面对照为主要研究材料,采用RAPD技术对二者的基因组DNA进行了扩增分析,并对部分差异片段进行了测序。结果发现:空间诱变的甜椒后代与其地面对照变异情况相比,SP4的基因组DNA发生了变异,共检测出8个多态性位点,在对照和变异体中各扩增出4个位点。对其中2条特异片段进行回收测序,序列同源性为97%。研究认为空间环境条件可以诱导甜椒后代植株基因组DNA发生变异。

To study effects of space environment on genomic DNA variation in the offsprings of sweet pepper,the 4th offspring and its ground control were used as materials.The genomic DNA variation was detected by random amplified polymprphic DNA（RAPD）,parts of the different fragments were sequenced.The results showed that the genomic DNA of SP4 after space mutation changed compared to the ground control.Eight polymorphic bands were detected.Four polymorphic bands were amplified in the ground control and variant separately.Two special fragments were sequenced and sequence homology was 97% .So space environment could induce the changes of the genomic DNA in the offsprings of sweet pepper.