叠氮化钠诱变对离体蝴蝶兰类原球茎生理的影响

EFFECT OF AZIDE SODIUM IN MUTAGENESIS ON PHYSIOLOGICAL TRAITS OF Phalaenosis PROTOCORM-LIKE BODY in vitro

以2、4、6和8mmol/L的叠氮化钠浸泡6h处理蝴蝶兰类原球茎后进行组织培养,测定了处理组和对照的蝴蝶兰类原球茎中脯氨酸、丙二醛和可溶性糖含量以及超氧化物歧化酶和过氧化氢酶的活力。结果表明,经叠氮化钠处理的类原球茎中的超氧化物歧化酶活力、脯氨酸和丙二醛含量均有上升,而过氧化氢酶活力与可溶性糖含量均有下降,并呈现浓度梯度效应。叠氮化钠诱变的半致死剂量介于4～8mmol/L之间,其中4mmol/L的叠氮化钠对蝴蝶兰类原球茎生理损伤较小,因此认为4mmol/L的叠氮化钠是诱变蝴蝶兰类原球茎较为合理的浓度。

The lethal and mutagenic effects of azide sodium on protocorm-like body（PLBs） of Phalaenosis was investigated.In these tests,PLBs of Phalaenosis were soaked for 6 hours in 2,4,6,8mmol /L azide sodium solution,and then inoculated to the culture medium.Proline,MDA,soluble sugar content,SOD and catalase activities of these cutured PLBs were measured.The results showed that,SOD activities,proline and MDA contents increased with the increase of azide sodium concentration,but catalase activities and soluble sugar content decreased.The half lethal dosage of azide sodium for Phalaenosis PLB mutagenesis was between 4 and 8mmol /L,and azide sodium at 4mmol /L had no significant physiological damage to PLBs of Phalaenosis.Thus,the optimal mutation dose for Phalaenosis PLB mutagenesis is 4mmol /L NaN3 and 6h soaking.