摘要
利用ERIC-PCR和16SrDNA全序列测定方法,研究了新疆棉田土壤中分离获得的58株固氮菌的遗传多样性及系统发育。采用平均连锁法(UPGMA)分析ERIC-PCR的聚类结果表明在Watson距离为0.65左右时可以将供试菌株分为9个大群。选取ERIC-PCR各群中代表菌株进行16SrRNA全序列测定分析,结果表明这些菌株分别属于Enterobacter、Bacillus、Acinetobacter、Pseudomonas、Serratia和Yersinia6个属。
To research the effect of nitrogen-fixing bacteria on grow of cotton, we used the technique of ERIC-PCR and 16S rDNA sequencing analysis to study genetic diversity of 58 nitrogen-fixing bacteria isolated from Tarim cotton soils in Xinjiang province. Cluster analysis was used for ERIC-PCR results of these strains by UPGMA. The results showed that the tested strains formed 9 clusters when the Waston distance was about 0.65. The typic strains of 9 clusters were identified as Enterobacter, Bacillus, Acinetobacter, Pseudomonas, Serratia, Yersinia by 16S rRNA sequencing analysis.
出处
《微生物学通报》
CAS
CSCD
北大核心
2010年第4期503-507,共5页
Microbiology China
基金
国家973计划前期研究专项项目(No.2007CB116303)
新疆生产建设兵团基础研究项目(No.2007JC06)
新疆生产建设兵团科技攻关计划项目(No.2006GG26)
