海水养殖含氮废水处理的复合微生态制剂研制

Developing Complex Probiotics Used in Treating Resoluble Nitrogen Wastewater from Seawater Culture

目前应用于海水养殖废水修复的微生态制剂不仅存在菌株针对性、适应性差的缺点,而且很少考虑功能微生物之间的相互作用,因此应用效果难以令人满意。本实验针对海水养殖过程中的氮素循环,选择分离自海水养殖废水环境的亚硝化细菌ZW38、反硝化细菌ZL5和蛋白酶产生菌ZS7进行配伍实验,以混合培养体系中各种类型细菌的数量及氨态氮、亚硝态氮和蛋白质的含量变化为指标,发现3种功能细菌以3:2:1的比例混合,且使用量为109CFU/L时,彼此之间可相互促进生长,经过5d处理,海水养殖废水中的主要污染物质可溶性蛋白和氨态氮的去除率分别达到94.0%和77.5%。

At the present time, probiotics used in recovering sea culture wastewater has some defects, such as no good counter, adaptation and relationship of these strains, so the applying effect is not satisfying. In our experiment, nitrosobacteria ZW38, denitrobacteria ZL5 and secreting proteinase strain ZS7, which were isolated from wastewater of marine culture, were mixed according the nitrogen cycle of seawater culture, the number of various strain and the content of NH4-N, NO2-N were determined. The results showed that these strains grow well each other when the ratio of ZW38, ZL5 and ZS7 is 3：2：1, and the total inoculating numer is 109 CFU/L. After 5 d for treating, the decomposed rate of resoluble protein and NH4-N is 94.0% and 77.5% respectively.