城市河道污水中好氧反硝化菌的分离鉴定与特性

Isolation and Characterization of Aerobic Denitrifier from the Sewage of Urban Rivers

采用富集培养和BTB(溴百里酚蓝)平板法从城市河道污水中筛选、分离获得了一株高效的好氧反硝化菌株ADZ1,48h内对硝酸盐的降解率为93.1%,总氮的去除率为34.7%。16SrRNA测序及系统发育分析结果表明该菌株属于Pseudamonas sp.,经VITEK2系统鉴定为Pseudomonas putida。对该菌株的反硝化特性进行了研究,结果表明,该菌株以乙醇为最佳碳源,在碳氮比达到12:1时,对硝酸盐的去除率达到98%以上,总氮去除率达到41.3%。该菌株对溶解氧、pH有着广泛的适应性,菌体活力强,有着良好的应用前景。

A bacterial strain ADZ1 that demonstrated higher efficient in the aerobic denitrification was isolated from the sewage of urban rivers on the selective medium, Bromothymol Blue （BTB）, after enriched in the shaking flasks. The reduction efficiency for nitrate and the total nitrogen reached 93.1% and 34.7% within 48 hours respectively. Sequence of 16S rRNA suggested that strain ADZ1 belongs to the genus of Pseudamonas, and further was identified as Pseudomonas putida by VITE K 2 systems. With the growth conditions that the ethanol served as the carbon source and C/N ratio was 12：1, the efficiency for nitrate and the total nitrogen reduction reached 98% and 41.3% respectively. Due to the broad adaptability to dissolved oxygen and pH, together with the high activity for aerobic denitrification, this strain showed the potential application in the future.