骨形态发生蛋白复合纤维蛋白载体修复骨缺损的实验研究

Studies on Segmental Bone Defect Repaired with Bone Morphogenetic Protein and Human Fibrin

目的：探讨以纤维蛋白作为骨形态发生蛋白（ＢＭＰ）的载体修复节段性骨缺损。材料和方法：于３６只新西兰白兔两侧桡骨干处造成１．５ｃｍ缺损，采用四种方法进行处理：Ａ组，植入２５ｍｇＢＭＰ与人纤维蛋白制成的复合物；Ｂ组，植入单纯２５ｍｇＢＭＰ；Ｃ组，植入单纯纤维蛋白；Ｄ组，留作空白对照。术后不同时间进行放射学、组织学和放射性核素显像检查。结果：Ａ组骨缺损区在成骨活跃程度、骨再生量和再生髓腔结构等方面均显著优于Ｂ组，使骨缺损得到了较彻底的修复。Ｃ、Ｄ两组均不能产生骨性愈合。结论：纤维蛋白是较理想的ＢＭＰ载体材料，以二者的复合物修复骨缺损可达到满意效果。

urpose: To repair segmental bone defect with the composite of bone morphogenetic protein(BMP) and human fibrin. Methods: Bone defects were created in 36 New Zealand rabbits on the bilateral radius and were treated with four kinds of implantations: A, Composites of 25 mg BMP and fibrin;B,25 mg BMP; C, fibrin alone; D, nothing as control. The defects were observed radiologically and histologically at different periods after operation and were examined by single photo emission computed tomography (SPECT) at 2, 4 and 8 weeks.Results: The defects treated with A implantation regenerated much more new bone and bridged earlier than the defects treated with B implantation,and were repaired satisfyingly 12 weeks after operation.The defects with C and D implantation couldn't get osseous tissue healing. Conclusion: The composites of BMP and fibrin can be used effectively to repair segmental bone defects.