摘要
应用细胞培养技术,在离体的正常和牙周病变根片上培养牙周膜细胞(PDLC),经24h和72h分别观察其各自的生长状态,并观察纤维粘连蛋白(FN)对细胞附着和生长的影响,通过细胞计数定量比较其附着和增殖程度的差异。结果显示,在离体的牙周病患牙根面上PDLC附着和增殖数量明显低于正常牙根面,细胞生长状态也较差;经FN作用后,其附着数量明显增加。进一步证实FN对PDLC确实是一种有效的促生长因子;此外,还提示FN的促附着和促增殖作用有所差异。
The purpose of this in vitro study was to observe and evaluate the effects of fibronectin(FN)on the growth of periodontal ligament cells(PDLC)on different root surfaces. Cell culture and scanning electronic microscopic techniques were used. The results showed that the growth pattern of PDLC on normal root surfaces were better than that of periodontitis. FN can significantly enhanced the attachment and proliferation of PDL cells on normal root surfaces.On the other hand, only the enhancement of attachment by FN was observed on the periodontitis root surfaces. It may be concluded that FN can promote the growth of PDLC and FN may play different effects on the attachment and proliferation of PDLC.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
1998年第2期111-113,I005,共4页
West China Journal of Stomatology
基金
国家自然科学基金
关键词
纤维粘连蛋白
牙周膜细胞
根面
牙周病
\ fibronectin\ \ periodontal ligament cell\ \ root surface